Reproduction – Complete NCERT Class 12 Biology Guide (Chapters 1, 2, 3)

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Unit 6 Reproduction - Complete NCERT Class 12 Biology Guide | All Chapters
📚 UNIT - 6

REPRODUCTION
जनन

Complete NCERT Guide for Class 12 Biology

NCERT-Based Content | CBSE | RBSE | Board Exam 2025-26
All Three Chapters with Summaries, Comparisons & Practice Questions

Chapter 1: Sexual Reproduction in Flowering Plants

Chapter 2: Human Reproduction

Chapter 3: Reproductive Health

Introduction to Unit 6: Reproduction

Reproduction is the biological process by which organisms produce new individuals of their own kind. It ensures the continuity of species and is one of the most fundamental characteristics of all living organisms.

Unit 6 of NCERT Class 12 Biology comprehensively covers reproduction across different life forms - from flowering plants to humans - and explores the various aspects of reproductive health.

Syllabus Coverage & Marks Distribution

Unit 6 - Reproduction: Chapter-wise Breakdown
Chapter No. Chapter Name Marks Weightage Key Topics
1 Sexual Reproduction in Flowering Plants 5 marks Flower structure, Pollination, Fertilization, Seed formation
2 Human Reproduction 4 marks Male & Female reproductive systems, Gametogenesis, Pregnancy
3 Reproductive Health 2 marks Contraception, STIs, Infertility, Population control
Total for Unit 6 11 marks 15-16% of Theory Paper

🎯 Board Exam Strategy: Unit 6 carries significant weightage (11 marks out of 70). Focus on diagrams, process descriptions, and comparative questions. Practice questions on pollination types, human reproductive processes, and contraceptive methods.

1

Sexual Reproduction in Flowering Plants 5 Marks

पुष्पी पादपों में लैंगिक प्रजनन

Chapter Overview

This chapter explores the fascinating process of sexual reproduction in angiosperms (flowering plants). It covers the complete reproductive cycle from flower development to seed and fruit formation, emphasizing the unique phenomenon of double fertilization found only in flowering plants.

Main Sections:

  • Structure of flower as reproductive organ
  • Stamens and pollen grain formation (Microsporogenesis)
  • Pistil and embryo sac formation (Megasporogenesis)
  • Types of pollination and their mechanisms
  • Double fertilization process
  • Post-fertilization changes: Endosperm, embryo, seed, and fruit development
  • Special reproductive methods: Apomixis and Polyembryony

🔑 Key Concepts to Remember

  1. Double Fertilization: Unique to angiosperms where one male gamete fuses with egg (syngamy) forming zygote, and another fuses with polar nuclei (triple fusion) forming triploid endosperm.
  2. Pollination Types: Self-pollination (autogamy, geitonogamy) vs Cross-pollination (xenogamy). Cross-pollination brings genetic variation.
  3. Outbreeding Devices: Mechanisms to prevent self-pollination and promote cross-pollination: Unisexuality (dioecy), Dichogamy (protandry, protogyny), Self-incompatibility, and Heterostyly. These ensure genetic variation.
  4. Pollen-Pistil Interaction: Only compatible pollen grains germinate on stigma. Incompatibility prevents self-pollination in many species.
  5. Endosperm: Nutritive tissue (3n) that nourishes developing embryo. Can be nuclear, cellular, or helobial type.
  6. Apomixis: Asexual reproduction through seeds without fertilization. Produces genetically identical offspring (clones).
  7. Polyembryony: Formation of multiple embryos in a single seed (e.g., Citrus, mango).

⚠️ Common Board Exam Questions:

  • Describe the process of double fertilization with diagram (5 marks)
  • Differentiate between autogamy and geitonogamy (2 marks)
  • Draw and label structure of mature embryo sac (3 marks)
  • Explain artificial hybridization technique in plants (3 marks)
2

Human Reproduction 4 Marks

मानव जनन

Chapter Overview

This chapter provides comprehensive coverage of human reproductive anatomy, physiology, and developmental processes. It explores both male and female reproductive systems, the formation of gametes, fertilization, pregnancy, and childbirth.

Main Sections:

  • Male reproductive system: Testes, accessory ducts, and glands
  • Female reproductive system: Ovaries, fallopian tubes, uterus
  • Gametogenesis: Spermatogenesis and Oogenesis
  • Menstrual cycle and hormonal regulation
  • Fertilization and implantation
  • Pregnancy, embryonic development, and placenta formation
  • Parturition (childbirth) and lactation

🔑 Key Concepts to Remember

  1. Spermatogenesis: Formation of sperms in seminiferous tubules. Starts at puberty, continues throughout life. One spermatogonium produces 4 functional sperms.
  2. Oogenesis: Formation of ova in ovaries. Begins in fetal life, arrests at birth, resumes at puberty. One oogonium produces one functional ovum and 3 polar bodies.
  3. Menstrual Cycle: 28-day cycle regulated by FSH, LH, Estrogen, and Progesterone. Phases: Menstrual, Follicular, Ovulatory, Luteal.
  4. Fertilization: Occurs in ampullary-isthmic junction of fallopian tube. Acrosome reaction helps sperm penetrate zona pellucida. Cortical reaction prevents polyspermy.
  5. Implantation: Blastocyst implants in endometrium around 7th day after fertilization. Trophoblast forms placenta.
  6. Placenta: Special tissue connecting fetus to mother. Acts as endocrine, nutritive, excretory, and respiratory organ. Secretes hCG, hPL, estrogen, progesterone.
  7. Parturition: Process of childbirth initiated by signals from fully developed fetus and placenta. Involves oxytocin and prostaglandin hormones.
  8. Lactation and Colostrum: Milk production starts after delivery. Colostrum (first milk) is rich in IgA antibodies providing passive immunity to newborn. Essential for infant's immune protection.

⚠️ Common Board Exam Questions:

  • Draw labeled diagram of human sperm / ovum (3 marks)
  • Describe the menstrual cycle with hormonal regulation (5 marks)
  • Differentiate between spermatogenesis and oogenesis (3 marks)
  • Explain the role of placenta during pregnancy (3 marks)
  • Describe the process of fertilization in humans (5 marks)
3

Reproductive Health 2 Marks

जनन स्वास्थ्य

Chapter Overview

This chapter addresses crucial aspects of reproductive health, including population control, contraception, sexually transmitted infections, and assisted reproductive technologies. It emphasizes the importance of reproductive health awareness in building a healthy society.

Main Sections:

  • Reproductive health: Problems and government strategies
  • Population explosion and need for birth control
  • Methods of contraception: Natural, Barrier, IUDs, Pills, Surgical
  • Medical Termination of Pregnancy (MTP) and legal aspects
  • Sexually Transmitted Infections (STIs): Types and prevention
  • Infertility and Assisted Reproductive Technologies (ART)

🔑 Key Concepts to Remember

  1. Contraceptive Methods: Natural (periodic abstinence, withdrawal), Barrier (condoms, diaphragm), IUDs (CuT, LNG-20), Oral pills, Surgical (vasectomy, tubectomy).
  2. Condoms: Only contraceptive method that protects against STIs including HIV/AIDS. Disposable and user-friendly.
  3. IUDs: Most widely accepted in India. Copper-releasing (suppress sperm motility), Hormone-releasing (prevent implantation). Effective for years.
  4. MTP Act 1971 & Amendment 2017: Legalized abortion with strict conditions. Opinion of one doctor required for MTP up to 12 weeks. For 12-20 weeks: two doctors. MTP Amendment Act 2017 extended upper limit to 20-24 weeks in special cases (fetal abnormalities) with opinion of two registered medical practitioners.
  5. STIs: Bacterial (Gonorrhea, Syphilis), Viral (Herpes, HIV/AIDS, HPV), Protozoan (Trichomoniasis), Fungal (Candidiasis). Most curable if detected early except HIV, Hepatitis-B, Herpes.
  6. ART Techniques: IVF-ET (test-tube babies), GIFT, ICSI, AI. Help infertile couples. First test-tube baby: Louise Joy Brown (1978).
  7. PCPNDT Act: Prohibits sex determination to prevent female feticide and declining sex ratio.

⚠️ Common Board Exam Questions:

  • Describe various contraceptive methods (5 marks)
  • What are STIs? Name causative agents and prevention (3 marks)
  • Differentiate between GIFT and IVF (2 marks)
  • What is MTP? When is it legally permitted? (3 marks)

Comparative Analysis of All Chapters

Table 1: Chapter-wise Comparison - Unit 6 Reproduction
Aspect Chapter 1
Flowering Plants
Chapter 2
Human
Chapter 3
Health
Focus Organism Angiosperms Humans Humans (Society)
Type of Study Plant reproduction Human anatomy & physiology Applied reproductive science
Key Organ Flower Reproductive system -
Unique Feature Double fertilization Menstrual cycle Contraception methods
Gametes Produced Pollen grains, Egg cell Sperms, Ovum -
Fertilization Site Embryo sac (ovule) Fallopian tube -
Product Formed Seed, Fruit Fetus, Baby -
Marks Weightage 5 marks 4 marks 2 marks
Diagram Importance High (TS ovary, embryo sac) Very High (reproductive systems) Medium
Application Focus Agriculture, Plant breeding Understanding human biology Social health, Population control

Unit-Level Important Concepts

Sexual Reproduction

✓ Involves gametes

✓ Genetic variation

✓ Two parents (usually)

✓ Meiosis involved

Examples: Flowering plants, Humans

Fertilization

✓ Fusion of gametes

✓ Restores diploid number

✓ Forms zygote

Types:

• External (fish, frogs)

• Internal (humans, plants)

Post-Fertilization

✓ Zygote development

✓ Embryo formation

✓ Protective structures

Products:

• Seeds (plants)

• Offspring (animals)

🎯 Cross-Chapter Connections

  • Fertilization: Double fertilization in plants vs Single fertilization in humans
  • Gamete Formation: Microsporogenesis/Megasporogenesis (plants) vs Spermatogenesis/Oogenesis (humans)
  • Reproductive Success: Pollination mechanisms (plants) vs Contraception (humans - preventing fertilization)
  • Challenges: Pollen-pistil incompatibility (plants) vs Infertility issues (humans)
  • Solutions: Artificial hybridization (plants) vs Assisted Reproductive Technologies (humans)

Practice Questions: Multiple Choice Questions

Instructions: Each question carries 1 mark. Choose the most appropriate answer.

Q1. Double fertilization is characteristic feature of:

  • (A) Gymnosperms
  • (B) Pteridophytes
  • (C) Angiosperms ✓
  • (D) Bryophytes
Correct Answer: (C) Double fertilization occurs only in angiosperms (flowering plants). One male gamete fuses with egg (syngamy) and another fuses with polar nuclei (triple fusion) forming triploid endosperm.

Q2. The nutritive tissue in angiosperm seed is:

  • (A) Embryo
  • (B) Endosperm ✓
  • (C) Perisperm
  • (D) Cotyledon
Correct Answer: (B) Endosperm is the triploid (3n) nutritive tissue formed after triple fusion. It nourishes the developing embryo.

Q3. Transfer of pollen grains from anther to stigma of same flower is called:

  • (A) Autogamy ✓
  • (B) Geitonogamy
  • (C) Xenogamy
  • (D) Allogamy
Correct Answer: (A) Autogamy is self-pollination within the same flower. Geitonogamy is transfer between flowers of same plant. Xenogamy is cross-pollination between different plants.

Q4. In human males, sperms are produced in:

  • (A) Epididymis
  • (B) Seminiferous tubules ✓
  • (C) Vas deferens
  • (D) Prostate gland
Correct Answer: (B) Spermatogenesis occurs in seminiferous tubules of testes. Epididymis stores sperms, vas deferens transports them.

Q5. How many sperms are formed from one primary spermatocyte?

  • (A) 1
  • (B) 2
  • (C) 4 ✓
  • (D) 8
Correct Answer: (C) One primary spermatocyte undergoes meiosis I to form 2 secondary spermatocytes, which undergo meiosis II to form 4 haploid spermatids that develop into 4 functional sperms.

Q6. The menstrual cycle is approximately of:

  • (A) 7 days
  • (B) 14 days
  • (C) 28 days ✓
  • (D) 40 days
Correct Answer: (C) The average human menstrual cycle is 28 days, though it can range from 21-35 days. Ovulation typically occurs around day 14.

Q7. Fertilization in humans occurs in:

  • (A) Ovary
  • (B) Fallopian tube ✓
  • (C) Uterus
  • (D) Cervix
Correct Answer: (B) Fertilization occurs in the ampullary-isthmic junction of the fallopian tube. The zygote then travels to uterus for implantation.

Q8. The hormone that confirms pregnancy is:

  • (A) Estrogen
  • (B) Progesterone
  • (C) hCG ✓
  • (D) FSH
Correct Answer: (C) Human Chorionic Gonadotropin (hCG) is secreted by placenta after implantation. Its detection in urine/blood confirms pregnancy.

Q9. Which contraceptive method protects from STIs?

  • (A) IUDs
  • (B) Oral pills
  • (C) Condoms ✓
  • (D) Tubectomy
Correct Answer: (C) Condoms are barrier methods that prevent direct contact, thus protecting from sexually transmitted infections including HIV/AIDS.

Q10. Copper-T is an example of:

  • (A) Barrier method
  • (B) IUD ✓
  • (C) Hormonal method
  • (D) Surgical method
Correct Answer: (B) Copper-T (CuT) is a copper-releasing intrauterine device. Copper ions suppress sperm motility and fertilizing capacity.

Q11. Vasectomy involves cutting of:

  • (A) Vas deferens ✓
  • (B) Fallopian tube
  • (C) Urethra
  • (D) Epididymis
Correct Answer: (A) Vasectomy is surgical sterilization in males where a small part of vas deferens is cut/tied to prevent sperm transport.

Q12. The first test-tube baby was born in:

  • (A) 1968
  • (B) 1978 ✓
  • (C) 1988
  • (D) 1998
Correct Answer: (B) Louise Joy Brown, the world's first test-tube baby, was born in 1978 through IVF-ET (In Vitro Fertilization and Embryo Transfer) technique.

Q13. Which is NOT a sexually transmitted infection?

  • (A) Gonorrhea
  • (B) Syphilis
  • (C) Typhoid ✓
  • (D) AIDS
Correct Answer: (C) Typhoid is caused by Salmonella typhi bacteria and spreads through contaminated food/water. It is not an STI. Gonorrhea, Syphilis, and AIDS are sexually transmitted.

Q14. Syphilis is caused by:

  • (A) Virus
  • (B) Bacterium ✓
  • (C) Protozoan
  • (D) Fungus
Correct Answer: (B) Syphilis is caused by the bacterium Treponema pallidum. It is curable with antibiotics if detected early.

Q15. GIFT stands for:

  • (A) Gamete Intra-Fallopian Transfer ✓
  • (B) Gamete Internal Fertilization Technique
  • (C) Gene Intra-Fallopian Transfer
  • (D) Gonad Implantation Fertilization Technique
Correct Answer: (A) GIFT is Gamete Intra-Fallopian Transfer where ovum and sperm are transferred into fallopian tube where fertilization occurs naturally.

Q16. The ploidy of endosperm in angiosperms is:

  • (A) Haploid (n)
  • (B) Diploid (2n)
  • (C) Triploid (3n) ✓
  • (D) Tetraploid (4n)
Correct Answer: (C) Endosperm is formed by triple fusion (one male gamete + two polar nuclei), hence it is triploid (3n).

Q17. Formation of seed without fertilization is called:

  • (A) Parthenocarpy
  • (B) Apomixis ✓
  • (C) Parthenogenesis
  • (D) Polyembryony
Correct Answer: (B) Apomixis is asexual reproduction through seeds without fertilization. It produces genetically identical offspring (clones).

Q18. Acrosome of sperm contains:

  • (A) Mitochondria
  • (B) Centriole
  • (C) Hydrolytic enzymes ✓
  • (D) Nucleus
Correct Answer: (C) Acrosome contains hydrolytic enzymes (hyaluronidase, acrosin) that help sperm penetrate zona pellucida of ovum during fertilization.

Q19. Implantation of embryo occurs in:

  • (A) Ovary
  • (B) Fallopian tube
  • (C) Uterus ✓
  • (D) Cervix
Correct Answer: (C) Blastocyst implants in the endometrium (inner lining) of uterus around 7th day after fertilization.

Q20. MTP stands for:

  • (A) Medical Termination of Pregnancy ✓
  • (B) Maternal Treatment Program
  • (C) Multiple Twin Pregnancy
  • (D) Medicinal Therapeutic Procedure
Correct Answer: (A) MTP is Medical Termination of Pregnancy (induced abortion). It was legalized in India in 1971 under strict conditions.

Short Answer Questions (2-3 Marks)

Q1. What is double fertilization? Why is it called so? 2M

Answer: Double fertilization is the process unique to angiosperms where two male gametes participate in two separate fusion events:
1. Syngamy: One male gamete fuses with egg cell to form diploid (2n) zygote
2. Triple fusion: Another male gamete fuses with two polar nuclei to form triploid (3n) endosperm
It is called "double" because fertilization occurs twice in the same embryo sac.

Q2. Differentiate between autogamy and geitonogamy. 2M

Answer:
Autogamy: Transfer of pollen grains from anther to stigma of the same flower. True self-pollination.
Geitonogamy: Transfer of pollen grains from anther of one flower to stigma of another flower on the same plant. Functionally cross-pollination but genetically self-pollination.

Q3. What is the significance of pollination? 2M

Answer: Pollination is essential for:
1. Transfer of pollen grains from anther to stigma
2. Enables fertilization to occur
3. Leads to seed and fruit formation
4. Cross-pollination brings genetic variation
5. Essential for crop production and plant reproduction

Q4. Distinguish between spermatogenesis and oogenesis. 3M

Answer:

SpermatogenesisOogenesis
Occurs in seminiferous tubules of testesOccurs in ovaries
Starts at puberty, continues lifelongBegins in fetal life, arrests at birth, resumes at puberty
One spermatogonium forms 4 functional spermsOne oogonium forms 1 ovum and 3 polar bodies
All products are equal in sizeUnequal division, ovum is larger

Q5. What is menstrual cycle? Name the hormones that regulate it. 3M

Answer: The menstrual cycle is a cyclic event occurring in sexually mature human females approximately every 28 days. It prepares the uterus for pregnancy.

Regulating hormones:
1. FSH (Follicle Stimulating Hormone): Stimulates follicle development
2. LH (Luteinizing Hormone): Triggers ovulation
3. Estrogen: Proliferation of endometrium
4. Progesterone: Maintains endometrium for implantation

Q6. What is the role of placenta during pregnancy? 3M

Answer: Placenta is a special tissue connecting fetus to mother through umbilical cord. Its roles:
1. Nutritive: Provides nutrients and oxygen from mother to fetus
2. Excretory: Removes waste products from fetal blood
3. Respiratory: Acts as lungs for fetus (O₂ and CO₂ exchange)
4. Endocrine: Secretes hCG, hPL, estrogen, progesterone
5. Barrier: Protects fetus from harmful substances (to some extent)

Q7. Name any four methods of contraception and their mode of action. 2M

Answer:
1. Condoms: Barrier method preventing sperm from meeting ovum
2. IUDs (CuT): Copper ions suppress sperm motility
3. Oral Pills: Hormones prevent ovulation by inhibiting FSH and LH
4. Vasectomy: Surgical method blocking vas deferens to prevent sperm transport

Q8. What are STIs? Name any two bacterial and two viral STIs. 2M

Answer: STIs (Sexually Transmitted Infections) are diseases transmitted through sexual contact.

Bacterial STIs:
1. Gonorrhea (caused by Neisseria gonorrhoeae)
2. Syphilis (caused by Treponema pallidum)

Viral STIs:
1. Genital Herpes (Herpes simplex virus)
2. AIDS (Human Immunodeficiency Virus - HIV)

Long Answer Questions (5 Marks)

Q1. Describe the process of double fertilization in flowering plants. 5M

Answer:
Double fertilization is unique to angiosperms and involves two fusion events:

Process:
1. Pollen Germination: Compatible pollen grain germinates on stigma forming pollen tube
2. Pollen Tube Growth: Tube grows through style guided by chemotropic signals, enters ovule through micropyle
3. Entry into Embryo Sac: Pollen tube enters embryo sac, releases two male gametes
4. First Fusion - Syngamy: One male gamete (n) fuses with egg cell (n) forming diploid zygote (2n). This develops into embryo.
5. Second Fusion - Triple Fusion: Another male gamete (n) fuses with two polar nuclei (n+n) forming triploid primary endosperm nucleus (3n). This develops into endosperm.

Significance:
- Ensures genetic diversity
- Endosperm provides nutrition to developing embryo
- Economical use of male gametes
- Confirms seed viability

Q2. Describe the structure and functions of human male reproductive system. 5M

Answer:
The male reproductive system consists of:

1. Testes (Primary Sex Organs):
- Paired oval structures located in scrotum
- Contain seminiferous tubules where spermatogenesis occurs
- Leydig cells produce testosterone hormone

2. Accessory Ducts:
- Rete testis: Network of tubules collecting sperms from seminiferous tubules
- Vasa efferentia: Transport sperms from rete testis to epididymis
- Epididymis: Stores and matures sperms
- Vas deferens: Muscular tube transporting mature sperms
- Urethra: Common passage for urine and semen

3. Accessory Glands:
- Seminal vesicles: Secrete fructose (energy for sperms), prostaglandins
- Prostate gland: Secretes alkaline fluid activating sperms
- Bulbourethral glands: Secrete lubricating mucus

4. External Genitalia:
- Penis: Male copulatory organ for insemination

Q3. Explain the menstrual cycle in human females with hormonal regulation. 5M

Answer:
The menstrual cycle is approximately 28-day cycle with four phases:

1. Menstrual Phase (Day 1-5):
- Shedding of endometrium with bleeding
- Low levels of all hormones
- Menstruation occurs

2. Follicular/Proliferative Phase (Day 6-13):
- FSH from pituitary stimulates follicle development
- Growing follicle secretes estrogen
- Estrogen causes proliferation and thickening of endometrium
- Uterine glands lengthen

3. Ovulatory Phase (Day 14):
- High estrogen triggers LH surge from pituitary
- LH surge causes rupture of Graafian follicle
- Release of secondary oocyte (ovulation)

4. Luteal/Secretory Phase (Day 15-28):
- Ruptured follicle transforms into corpus luteum
- Corpus luteum secretes progesterone (and some estrogen)
- Progesterone maintains endometrium for implantation
- If fertilization doesn't occur, corpus luteum degenerates
- Progesterone and estrogen levels fall
- Endometrium breaks down → Menstruation begins

Hormonal Feedback:
- High estrogen → Inhibits FSH (negative feedback)
- High estrogen (mid-cycle) → Stimulates LH surge (positive feedback)
- Progesterone → Inhibits LH and FSH

Q4. Describe various contraceptive methods available for humans. 5M

Answer:
Contraceptive methods can be classified as follows:

1. Natural Methods:
- Periodic Abstinence: Avoiding coitus during ovulation period (day 10-17). High failure rate.
- Withdrawal: Male withdraws before ejaculation. High failure rate.
- Lactational Amenorrhea: Effective for 6 months after delivery during intense lactation.

2. Barrier Methods:
- Condoms: Latex sheaths covering penis/vagina. Protect from STIs. Disposable.
- Diaphragm/Cervical Cap: Cover cervix, block sperm entry. Reusable.
- Spermicides: Chemicals killing sperms. Used with barriers.

3. Intrauterine Devices (IUDs):
- Non-medicated: Lippes loop - increase phagocytosis
- Copper-releasing: CuT, Cu7 - suppress sperm motility
- Hormone-releasing: Progestasert, LNG-20 - prevent implantation. Effective for 5 years.

4. Oral Contraceptives (Pills):
- Contain progestogens ± estrogens
- Prevent ovulation by inhibiting FSH and LH
- Taken for 21 days, 7 days gap
- Emergency pills within 72 hours of unprotected coitus

5. Surgical Methods (Sterilization):
- Vasectomy: Cutting/tying vas deferens in males
- Tubectomy: Cutting/tying fallopian tubes in females
- Highly effective but irreversible

Ideal Contraceptive Qualities: User-friendly, easily available, effective, reversible, minimal side effects, doesn't interfere with sexual act.

Q5. What are Assisted Reproductive Technologies? Describe any three techniques. 5M

Answer:
Assisted Reproductive Technologies (ART) are techniques to help infertile couples have children.

Major ART Techniques:

1. IVF-ET (In Vitro Fertilization and Embryo Transfer):
- Ova from wife/donor and sperms from husband/donor collected
- Fertilization occurs in laboratory (in vitro) under controlled conditions
- Embryo formed (8-blastomere stage or blastocyst)
- Embryo transferred to uterus (IUT) or fallopian tube (ZIFT)
- Babies born are called "test-tube babies"
- First test-tube baby: Louise Joy Brown (1978)

2. GIFT (Gamete Intra-Fallopian Transfer):
- Ovum and sperms collected after inducing superovulation
- Both gametes transferred directly into fallopian tube
- Fertilization occurs naturally inside fallopian tube
- Embryo travels to uterus for implantation
- Difference from IVF: Fertilization occurs in vivo (inside body)

3. ICSI (Intra-Cytoplasmic Sperm Injection):
- Special type of IVF
- Single sperm directly injected into ovum cytoplasm
- Used when male has very low sperm count or motility
- Embryo formed in lab, then transferred to uterus
- Overcomes severe male infertility

Other techniques:
- AI (Artificial Insemination): Semen artificially introduced into vagina/uterus
- Surrogate mother: Embryo transferred to another woman who carries pregnancy
- Donor gametes: Used when couple cannot produce viable gametes

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